endonuclease

简明释义

英[ˌendəʊˈnjuːklɪˌeɪz]美[ˌendoˈnʊkliˌes;ˌendoˈnʊkliˌe

n. [生化] 核酸内切酶

英英释义

单词用法

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例句

1.Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.

结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。

2.Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.

方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。

3.Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.

结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。

4.The core technology in TILLING is screening mutant library by using single-strand endonuclease.

TILLING技术的核心是使用单链核酸内切酶大规模筛选突变体库。

5.In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.

本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。

6.The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.

建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。

7.Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.

方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。

8.The structure of vector was identified by endonuclease analyzing and base sequencing.

通过核酸内切酶酶切分析及测序鉴定载体结构。

9.The enzyme endonuclease plays a crucial role in DNA repair mechanisms.

酶内切酶在DNA修复机制中起着至关重要的作用。

10.Researchers used endonuclease to cut DNA at specific sites for gene editing.

研究人员使用内切酶在特定位置切割DNA以进行基因编辑。

11.In molecular biology, endonuclease is essential for cloning procedures.

在分子生物学中，内切酶对克隆程序至关重要。

12.The function of endonuclease can be inhibited by certain chemicals.

某些化学物质可以抑制内切酶的功能。

13.Certain types of endonuclease are used in recombinant DNA technology.

某些类型的内切酶用于重组DNA技术。

作文

In the realm of molecular biology, the role of enzymes is paramount, particularly when it comes to DNA manipulation and repair. One such enzyme that has garnered significant attention is endonuclease. An endonuclease (内切酶) is a type of enzyme that cleaves the phosphodiester bond within a nucleic acid strand, which can be either DNA or RNA. This ability to cut nucleic acids at specific sites makes endonuclease a vital tool in genetic engineering and biotechnology. The function of endonuclease is crucial for various cellular processes. For instance, during DNA replication, if there are any errors or damage in the DNA strand, endonuclease can recognize these faults and initiate a repair process. By cutting the damaged segment, it allows other enzymes to remove the faulty nucleotides and replace them with the correct ones. This action is essential for maintaining the integrity of genetic information within the cell. Moreover, endonuclease plays a pivotal role in the process of apoptosis, or programmed cell death. During apoptosis, cells undergo a series of controlled steps leading to their self-destruction. endonuclease facilitates this process by fragmenting the DNA, ensuring that the cell dismantles itself in an orderly fashion. This is important for preventing uncontrolled cell growth, which can lead to cancer. In biotechnology, endonuclease is extensively utilized in cloning and gene editing. Techniques such as CRISPR-Cas9 rely on the precision of endonuclease to create targeted cuts in the DNA, allowing scientists to insert, delete, or modify genes with high accuracy. This capability opens up new avenues for research and therapy, particularly in treating genetic disorders. Different types of endonuclease exist, each with unique characteristics and specificities. For example, restriction endonucleases, commonly used in molecular cloning, can recognize specific sequences of DNA and cut at those sites. This property enables researchers to manipulate DNA fragments effectively, facilitating the construction of recombinant DNA molecules. Additionally, understanding the mechanisms by which endonuclease operates can lead to advancements in medical science. For instance, if we can harness the power of endonuclease to target and destroy viral DNA within infected cells, it could pave the way for innovative treatments for viral infections. In summary, endonuclease (内切酶) is an indispensable enzyme in molecular biology, playing critical roles in DNA repair, apoptosis, and biotechnological applications. Its ability to cleave nucleic acids at precise locations has not only advanced our understanding of genetic processes but also revolutionized the field of genetic engineering. As research continues to uncover the complexities of endonuclease and its various forms, we can anticipate even more groundbreaking developments in genetics and medicine in the future.