isoelectric

简明释义

英[ˌaɪsəʊɪˈlektrɪk]美[ˌaɪsoʊɪˈlektrɪk]

adj. 等电位的；[电] 零电位差的

英英释义

单词用法

同义词

反义词

例句

1.Some similarities in polypeptide components, molecular weight, isoelectric point and amino acid composition were found, but no immunocross reaction between them was observed.

发现两者在蛋白质组成，分子量，等电点及氨基酸组成上颇为相近。但免疫扩散试验证实两者无免疫交叉反应。

2.Using this method, clinical research is carried out in 32 cases with the spatial distribution of isoelectric map of five types of abnormal ECG. Some inspiring results are obtained.

利用该方法作者以32例临床病例对五种异常心电信号进行了研究，得到了一些启发性结果。

3.While for the adsorption kinetics of Hb and lysozyme which with higher isoelectric points, little electrostatic hindrance effects were suffered.

而对于具有较高等电点的血红蛋白和溶菌酶的吸附动力学，却几乎不受静电排阻作用的影响。

4.Its isoelectric points are 3.85 and 4.35, determined by iso-eletric focusing electrophoresis.

用薄层聚丙烯酰胺凝胶等电聚焦电泳测得该酶的等电点为3.85和4.35；

5.After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.

定量后，分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。

6.The content of casein can be used as a index of quality for the milk, which can be determined by the method of isoelectric precipitation.

牛奶中酪蛋白含量是衡量牛奶质量的一个重要指标，采用蛋白质等电沉淀法可测定其含量。

7.Its molecular weight is 64,000 and isoelectric point around pH 4.2 as determined by SDS-PAGE and isoelectric focusing analysis respectively.

SDS聚丙烯酰胺凝胶电泳测定分子量为64,000。等电聚焦电泳测得等电点在4.2左右。

8.Objective: to determine the amino acid composition and isoelectric point of porcine platelet-derived growth factor.

目的：测定猪血小板衍生生长因子的氨基酸组成和等电点。

9.In protein electrophoresis, the sample is separated based on its isoelectric 等电 point.

在蛋白质电泳中，样品根据其等电点进行分离。

10.The isoelectric 等电 focusing technique is essential for purifying proteins.

等电聚焦技术对蛋白质的纯化至关重要。

11.During the experiment, we measured the pH at which the protein became isoelectric 等电.

在实验中，我们测量了蛋白质变为等电的pH值。

12.Understanding the isoelectric 等电 point helps in optimizing protein crystallization.

理解等电点有助于优化蛋白质结晶。

13.The isoelectric 等电 state of a molecule can affect its solubility in various solvents.

分子的等电状态会影响其在各种溶剂中的溶解度。

作文

The concept of isoelectric refers to a state in which a molecule or a system has no net electric charge. This term is commonly used in various scientific fields, including chemistry and biology, to describe the condition of proteins, amino acids, and other charged species. Understanding the isoelectric point of a substance is crucial for numerous applications, such as protein purification, electrophoresis, and drug formulation. In chemistry, the isoelectric point (pI) of an amino acid is the pH at which the amino acid carries no net charge. At this specific pH, the positive and negative charges balance each other out, making the molecule neutral. For instance, if we consider the amino acid glycine, it has both an amino group (-NH2) and a carboxyl group (-COOH). At a certain pH, the carboxyl group will lose a proton, becoming negatively charged, while the amino group will gain a proton, becoming positively charged. The isoelectric point of glycine occurs at a pH of around 6.0, where it exists predominantly in its zwitterionic form, balancing the positive and negative charges. In biological systems, the isoelectric point is particularly important for proteins. Proteins are composed of various amino acids, each with its own pI. The overall isoelectric point of a protein can be determined by the composition of its constituent amino acids. When the pH of the surrounding environment matches the protein's isoelectric point, the protein becomes less soluble in solution, which can lead to precipitation. This property is exploited in techniques such as isoelectric focusing, a method used to separate proteins based on their pI values. Moreover, the isoelectric concept extends beyond just amino acids and proteins. It is also relevant in the field of biochemistry when discussing nucleic acids. For example, DNA molecules can have an isoelectric point that influences their behavior in an electric field during gel electrophoresis. Understanding the isoelectric point of nucleic acids can help researchers manipulate and analyze these molecules more effectively. In drug formulation, the isoelectric point of a drug molecule can affect its solubility and absorption in the body. Drugs that are charged at physiological pH may have different absorption rates compared to their neutral counterparts. Therefore, knowing the isoelectric characteristics of a drug can assist pharmaceutical scientists in designing more effective medications. In summary, the term isoelectric is a fundamental concept in science that describes a state of neutrality in terms of electric charge. Whether it pertains to amino acids, proteins, or nucleic acids, understanding the isoelectric point is essential for various applications in research and industry. By mastering this concept, scientists can better manipulate biological molecules for therapeutic and analytical purposes, ultimately contributing to advancements in medicine and biotechnology. The implications of isoelectric properties are vast, demonstrating the interconnectedness of charge, structure, and function in the molecular world.

“等电”的概念指的是一种状态，其中分子或系统没有净电荷。这个术语在化学和生物学等多个科学领域中被广泛使用，用于描述蛋白质、氨基酸和其他带电物质的状态。理解某种物质的等电点对于许多应用至关重要，例如蛋白质纯化、电泳和药物配方。 在化学中，氨基酸的等电点（pI）是指氨基酸携带净电荷为零的pH值。在这个特定的pH值下，正负电荷相互平衡，使分子呈中性。例如，如果我们考虑氨基酸甘氨酸，它既有氨基（-NH2），也有羧基（-COOH）。在某个特定的pH值下，羧基会失去一个质子，变成带负电荷，而氨基会获得一个质子，变成带正电荷。甘氨酸的等电点发生在大约pH 6.0，此时它主要以其两性离子形式存在，平衡正负电荷。 在生物系统中，等电点对蛋白质尤为重要。蛋白质由多种氨基酸组成，每种氨基酸都有自己的pI。蛋白质的整体等电点可以通过其组成氨基酸的构成来确定。当周围环境的pH值与蛋白质的等电点相匹配时，蛋白质在溶液中的溶解度降低，这可能导致沉淀。这一特性在等电聚焦等技术中得到了利用，这是一种根据pI值分离蛋白质的方法。 此外，等电的概念不仅限于氨基酸和蛋白质。在生物化学领域讨论核酸时也相关。例如，DNA分子可以具有等电点，这会影响它们在电场中的行为，特别是在凝胶电泳过程中。理解核酸的等电点可以帮助研究人员更有效地操控和分析这些分子。 在药物配方中，药物分子的等电点可能影响其在体内的溶解度和吸收率。在生理pH下带电的药物与中性药物相比，可能具有不同的吸收速率。因此，了解药物的等电特性可以帮助制药科学家设计更有效的药物。 总之，术语等电是科学中的一个基本概念，描述了电荷中性的状态。无论是涉及氨基酸、蛋白质还是核酸，理解等电点对于研究和工业中的各种应用都是至关重要的。通过掌握这一概念，科学家们可以更好地操控生物分子，以用于治疗和分析目的，最终推动医学和生物技术的进步。等电特性的影响广泛，展示了分子世界中电荷、结构和功能之间的相互关联。