luciferase

简明释义

英[ˈluːsɪfərˌeɪs]美[luːˈsɪfəˌreɪs]

n. [生化] 荧光素酶

英英释义

单词用法

同义词

反义词

例句

1.The transcriptional activity of the two splicing variants was detected with a luciferase reporter in transient transfections of human embryonic kidney293T cells.

瞬时转染人胚胎肾细胞293t，用荧光素酶报告基因检测这两种剪切形式的转录活性。

2.The human GRP94 promoter was then cloned upstream of the luciferase gene and showed enhanced activity in hypoxic conditions.

随后人GRP94启动子克隆了荧光素酶基因上游区且缺氧环境中活性增强。

3.All three mutants showed higher luciferase activity than that of the wild type, and the V85I mutant showed the highest activity.

三个突变异种均显示比野生型具有更高的荧光素酶活性，并且V85I突变异种活性最强。

4.In in vitro, DHT increased the AR activity and induced the expression of luciferase reporter gene in a concentration-dependent manner.

核净未能引起荧光素酶活性值的增加，但菌核净能抑制DHT依赖的AR活性，并呈现。

5.Objective The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.

目的探讨感光受体外周蛋白结合蛋白（PBP）在体外是否有促进变性蛋白质复性的作用。

6.We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.

我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。

7.The investigators used genetically engineered E. coli cells to produce luciferase as a function of AHL concentration.

研究者用基因工程的大肠埃希菌细胞生产荧光素酶作为AHL浓度函数。

8.Researchers use luciferase 荧光素酶 in bioluminescent assays to measure gene expression.

研究人员在生物发光测定中使用荧光素酶 luciferase 来测量基因表达。

9.The luciferase 荧光素酶 enzyme is commonly found in fireflies, giving them their characteristic glow.

该荧光素酶 luciferase 酶通常存在于萤火虫中，使它们发出特有的光芒。

10.Scientists have engineered a new type of luciferase 荧光素酶 that emits light in the near-infrared range.

科学家们已经设计了一种新的荧光素酶 luciferase，它在近红外范围内发光。

11.Using luciferase 荧光素酶 as a reporter gene allows for easy tracking of cellular processes.

使用荧光素酶 luciferase 作为报告基因可以轻松跟踪细胞过程。

12.The application of luciferase 荧光素酶 in drug discovery has revolutionized the way we screen compounds.

在药物发现中应用荧光素酶 luciferase 已经彻底改变了我们筛选化合物的方式。

作文

Luciferase is an enzyme that plays a crucial role in bioluminescence, the natural phenomenon where living organisms produce light. This fascinating process can be observed in various species, including fireflies, certain types of fungi, and deep-sea creatures. The name 'luciferase' comes from the Latin word 'lucifer,' meaning 'light-bringer.' The study of luciferase is not just limited to understanding how these organisms emit light; it has significant implications in various scientific fields, including molecular biology and biotechnology. In molecular biology, luciferase is often used as a reporter gene. Scientists insert the gene that codes for luciferase into the DNA of other organisms to track biological processes. When the target gene is activated, the luciferase enzyme produces light, which can be measured. This allows researchers to visualize and quantify gene expression, making it an invaluable tool in genetic research and drug development. The application of luciferase extends beyond basic research. In the field of medicine, it is used in assays to detect the presence of specific molecules or cells. For example, luciferase-based assays can identify cancer cells in a sample by measuring the light emitted when these cells are present. This method is not only sensitive but also allows for real-time monitoring of cellular activities, providing insights into disease progression and treatment efficacy. Moreover, luciferase is being explored for use in imaging techniques. Researchers are developing methods to utilize luciferase in live animal imaging, which could revolutionize how scientists study diseases in vivo. By introducing luciferase into specific tissues or cells, they can track the spread of cancer or the effect of therapies in real-time, offering a dynamic view of biological processes. The potential of luciferase is immense, yet its use also raises ethical considerations. As we harness the power of this enzyme for various applications, we must consider the implications of genetically modifying organisms. While the benefits of such technologies are significant, the long-term effects on ecosystems and biodiversity remain uncertain. Therefore, responsible research practices and regulatory frameworks are essential to ensure that the use of luciferase and similar technologies is safe and sustainable. In conclusion, luciferase is more than just an enzyme that produces light; it is a powerful tool in modern science. Its applications in molecular biology, medicine, and imaging highlight its versatility and importance. As research continues to advance, the understanding and utilization of luciferase will undoubtedly lead to groundbreaking discoveries and innovations that could benefit humanity. However, with great power comes great responsibility, and it is imperative that we proceed with caution as we explore the depths of bioluminescence and its potential applications.

荧光素酶是一种在生物发光中起着至关重要作用的酶，这是一种自然现象，生物体能够产生光。这一迷人的过程可以在各种物种中观察到，包括萤火虫、某些类型的真菌和深海生物。‘荧光素酶’这个名字来源于拉丁文‘lucifer’，意为‘带光者’。对荧光素酶的研究不仅限于理解这些生物如何发出光；它在分子生物学和生物技术等多个科学领域具有重要意义。 在分子生物学中，荧光素酶常用作报告基因。科学家将编码荧光素酶的基因插入其他生物的DNA中，以跟踪生物过程。当目标基因被激活时，荧光素酶酶会产生光，可以被测量。这使得研究人员能够可视化和量化基因表达，使其成为遗传研究和药物开发中不可或缺的工具。 荧光素酶的应用超越了基础研究。在医学领域，它被用于检测特定分子或细胞的测定。例如，基于荧光素酶的测定可以通过测量这些细胞存在时发出的光来识别样本中的癌细胞。这种方法不仅灵敏，而且允许实时监测细胞活动，为疾病进展和治疗效果提供了深入见解。 此外，荧光素酶正在被探索用于成像技术。研究人员正在开发利用荧光素酶进行活体动物成像的方法，这可能会彻底改变科学家研究体内疾病的方式。通过将荧光素酶引入特定组织或细胞，他们可以实时跟踪癌症的扩散或治疗的效果，从而提供生物过程的动态视图。 荧光素酶的潜力是巨大的，但其使用也引发了伦理考虑。当我们利用这种酶的力量进行各种应用时，我们必须考虑基因改造生物体的影响。虽然这些技术的好处是显著的，但对生态系统和生物多样性的长期影响仍然不确定。因此，负责任的研究实践和监管框架对于确保荧光素酶及类似技术的使用是安全和可持续的至关重要。 总之，荧光素酶不仅仅是一种产生光的酶；它是现代科学中的一种强大工具。它在分子生物学、医学和成像中的应用突显了它的多功能性和重要性。随着研究的不断进展，对荧光素酶的理解和利用无疑将导致突破性的发现和创新，这些发现和创新可能会造福人类。然而，强大的力量伴随着巨大的责任，在我们探索生物发光及其潜在应用的深度时，务必要谨慎行事。