lysate

简明释义

英[ˈlaɪseɪt]美[ˈlaɪseɪt]

n. 溶菌产物；溶解产物

英英释义

单词用法

同义词

反义词

例句

1.Methods Tachypleus amebocyte lysate from two manufacturers was used to investigated the interference test of three batches of enoxacin gluconate injection.

方法：用不同厂家的鲎试剂对不同批号的丹芪注射液分别进行干扰试验，考察丹芪注射液细菌内毒素检测法。

2.To explore if the method comprising Limulus amoebocyte lysate (LAL) test and enumeration of total Gram-negative bacteria (GNB) can be used for screening of irradiated chicken.

探讨内毒素检测(LAL)与革兰氏阴性菌计数(GNB)相结合的方法应用于辐照鸡肉的筛选的可能性。

3.Tachypleus amebocyte lysate test for bacterial endotoxins in Aceglutamide and sodium chloride injection;

应用动态浊度法鲎试验定量测定复方苦参注射液中细菌内毒素含量。

4.Thus, quick preparation of the histiocyte lysate can be realized.

实现了快速制备组织细胞裂解液。

5.The results obtained by the limulus amebocyte lysate test were the same with those from the biosensor technique.

鲎试验结果与生物传感器测定结果基本一致。

6.Cell debris in the sheared lysate is then cleared by sedimentation and protein–DNA complexes are selectively immunoprecipitated using specific antibodies to the protein(s) of interest.

细胞碎片在修剪得溶菌液可以用沉降和蛋白-DNA复合体，是使用特定抗体到指定蛋白的选择。

7.When laminarin as an immune activator was present, phenoloxidase (PO) activities in cell debris, hemocyte lysate solution and plasma were measured as 26.80, 16.68, and 2.53 u, respectively.

在免疫激活剂昆布多糖存在下，分别测得小菜蛾幼虫血细胞碎片、血细胞裂解液和血浆的酚氧化酶活性为26.80U, 16.68 U和2.53 U。

8.OBJECTIVE to approach the feasibility of replacing pyrogen test with bacterial endotoxin test (BET) by analysing the interference of the injection to Limulus Amebocyte Lysate (LAL).

目的：分析研究大蒜注射液对鲎试剂的干扰作用，探讨以细菌内毒素检查法取代热原检查法检测大蒜注射液的可行性。

9.The researcher prepared a lysate for the protein analysis.

研究人员准备了一个裂解液用于蛋白质分析。

10.After centrifugation, the lysate was collected for further study.

离心后，收集了裂解液以便进一步研究。

11.The lysate contains various cellular components that can be analyzed.

该裂解液包含可以分析的各种细胞成分。

12.We used a bacterial lysate to stimulate the immune response in our experiments.

我们使用了细菌裂解液来刺激实验中的免疫反应。

13.The lysate was subjected to electrophoresis to separate proteins.

该裂解液经过电泳以分离蛋白质。

作文

In the field of biology and biochemistry, the term lysate refers to a solution that contains the contents of lysed cells. This process of lysis involves breaking down the cell membrane, which can be achieved through various methods such as physical disruption, chemical treatment, or enzymatic action. The resulting lysate is rich in proteins, nucleic acids, and other cellular components that are crucial for scientific research and applications. Understanding the significance of lysate in laboratory settings is essential for researchers who aim to study cellular processes, protein interactions, or genetic material. For instance, when scientists wish to analyze specific proteins, they can create a lysate from a culture of cells expressing those proteins. By lysing the cells, they release the proteins into the solution, allowing for further purification and analysis. Moreover, lysate plays a vital role in various biotechnological applications. In the production of vaccines, for example, viral lysates may be used to stimulate an immune response. By using the components derived from lysed viruses, researchers can develop vaccines that help protect against infectious diseases. This process underscores the importance of lysate in public health and disease prevention. Additionally, the use of lysate extends to molecular biology techniques such as Western blotting and PCR (Polymerase Chain Reaction). In Western blotting, proteins within a lysate are separated by gel electrophoresis and then transferred to a membrane for detection. This technique allows scientists to identify specific proteins and study their expression levels under various conditions. On the other hand, when working with DNA or RNA, researchers often prepare a lysate to extract these nucleic acids for further analysis. The integrity and quality of the lysate are crucial, as contaminants can interfere with downstream applications, leading to inaccurate results. Furthermore, the preparation of a lysate must be performed carefully to ensure that the cellular components remain intact and functional. Different types of cells may require specific lysis buffers or conditions to achieve optimal results. For example, bacterial lysates might be prepared using lysozyme, while mammalian cells may require detergents to disrupt their membranes effectively. In conclusion, the concept of lysate is fundamental in biological research and biotechnology. It serves as a valuable tool for extracting and analyzing the intricate components of cells, enabling scientists to gain insights into cellular functions and develop innovative solutions in medicine and industry. As our understanding of biology continues to evolve, the importance of lysate will undoubtedly remain significant, paving the way for future discoveries and advancements in science.

在生物学和生物化学领域，术语lysate指的是含有溶解细胞内容物的溶液。溶解过程涉及破坏细胞膜，这可以通过物理破坏、化学处理或酶促作用等多种方法实现。生成的lysate富含蛋白质、核酸和其他细胞成分，这些成分对于科学研究和应用至关重要。 理解lysate在实验室环境中的重要性，对于希望研究细胞过程、蛋白质相互作用或遗传物质的研究人员来说是必不可少的。例如，当科学家希望分析特定蛋白质时，他们可以从表达这些蛋白质的细胞培养物中创建lysate。通过裂解细胞，他们将蛋白质释放到溶液中，从而允许进一步的纯化和分析。 此外，lysate在各种生物技术应用中也发挥着至关重要的作用。在疫苗生产中，例如，病毒lysates可能被用来刺激免疫反应。通过使用从溶解病毒中提取的成分，研究人员可以开发出帮助预防传染病的疫苗。这一过程强调了lysate在公共卫生和疾病预防中的重要性。 此外，lysate的使用还扩展到分子生物学技术，如西方印迹法和PCR（聚合酶链反应）。在西方印迹法中，lysate中的蛋白质通过凝胶电泳分离，然后转移到膜上进行检测。这一技术使科学家能够识别特定蛋白质并研究其在不同条件下的表达水平。 另一方面，在处理DNA或RNA时，研究人员通常准备lysate以提取这些核酸以便进一步分析。lysate的完整性和质量至关重要，因为污染物可能干扰后续应用，导致结果不准确。 此外，准备lysate必须谨慎进行，以确保细胞成分保持完整和功能。不同类型的细胞可能需要特定的裂解缓冲液或条件以获得最佳结果。例如，细菌lysates可能需要使用溶菌酶，而哺乳动物细胞则可能需要去污剂有效地破坏其膜。 总之，lysate的概念在生物研究和生物技术中是基础性的。它作为提取和分析细胞复杂成分的宝贵工具，使科学家能够深入了解细胞功能，并在医学和工业领域开发创新解决方案。随着我们对生物学的理解不断发展，lysate的重要性无疑将继续显著，为未来的发现和科学进步铺平道路。